LSM 800

Fully Motorized and Computer controlled Inverted Fluorescence Research Microscope

Model: Axio Observer.Z1
Axio Observer.Z1 stand incl.
– motorized focus drive (smallest increment 10 nm),  Z-drive fine
operation flat on left side, – TFT touchscreen,-
– Path deflection to the tube,  Sideport 60N L/R 100, 3 switching
positions for attachment of LSM and cameras,
– tiltable carrier for transmitted-light illumination, equipped with
luminous-field diaphragm, 3-position filter changer and Interface for motorized
condenser incl. Shutter.
– motorized 6-position objective nosepiece for transmitted light
brightfield and DIC,
Binocular tube 45° / field of view 23mm in intermediate image plane,
2x eyepiece PL 10x/23 Br. foc., 2x eyepiece eyecup,

Motorized XY-scanning stage

Scanning Stage 130×100 STEP Set for LSM

Scanning Stage 130×100 STEP Set for LSM consists of:
– Scanning Stage 130×100 STEP with stepper motor (2 mm spindle pitch)
incl. stepper controller SMC 2009, Joystick XY, fixed motor cable with
exit on stage left back side and universal mounting frame K (160 mm x 110 mm),
also suitable for Z-Piezo
– travel range: 130 mm x 100 mm (adjustable)
– max. speed: 50 mm/s
– resolution: 0.1 µm
– reproducibility: +/- 1 µm
– absolute accuracy: +/- 5 µm
– stage surface: 325 mm x 144 mm

Objectives and DIC attachment

Objective “Plan-Apochromat” 10x/0.45 M27 (WD=2.1mm)

Objective Plan-Apochromat 20x/0.8 M27

Objective Plan-Apochomat 40x/1.3 Oil DIC M27
(WD=0.2mm), (UV)VIS-IR,

Objective Plan-Apochromat 63x/1.4 Oil DIC M27 (WD=0.19mm), incl. Immersol 518 F for 30 °C, oiler 20ml

DIC Attachment

DIC slider Planapo 10x/0.45 II

DIC slider PA 20x/0.80 II

DIC slider PA 40x/1.3 III

DIC slider PA 63x/1.40 III

Epi-fluorescence equipment

Reflected, fluorescence light Set for LSM 800 consists of:
– reflected-light illumination fluorescence beam path with internal shutter and reception for sliders, incl. Iris stop slider for incident-light equipment adjustable in discrete steps, to be used as fluorescence attenuator
– apochromatic corrected beam path with transmission >50% from 340 to 700nm
– motorized 6-position reflector turret for P&C modules (e.g. fluorescence filter sets) with a switching time of 200ms
– Antiglare screen LSM

Reflector module FL EC P&C

Filter set 49 DAPI shift free

Filter set 10 GFP / FITC / Cy2 shift free

Filter set 20 TRITC / Cy3 / Rhodamin shift free

Illuminator HXP 120 V

LSM 800 Spectral confocal laser scanning module with 2MA – PMT + 1 GaAsP spectral detection including diode laser 405, 488, 561 & 640 nm with ZEN 2.3 software

LSM 800 Confocal MA-detection module

LSM 800 Confocal MA-detection module consists of:
– LSM 800 scan module multialkali PMT (MA-PMT) Confocal point scanning system with patented long pass (LP) variable secondary dichroic (VSD) for flexible spectral imaging and two sensitive MA-PMT detectors. Patented linear scanning with independently driven galvanometric scanners for quantitative imaging. Up to 8 frames/s at 512 x 512 pixel. Continuous zoom optics from 0.5x to 40x. Maximum frame resolution 6000 x 6000 pixel (with Airyscan 4000 x 4000 pixel) adjustable for each axis.12.7x 12.7mm² field of view in intermediate image plane. Variable pinhole with automatic alignment.Main beam splitter (MBS) for 4 laser lines  (405, 488, 561 and 640nm).

– LSM 800 Laser module LM URGB with fiber-coupled, pigtailed and collimated lasers. Following lasersare available:
– Diode laser 405nm, 5mW, laser class 3B
– Diode laser 488nm, 10mW, laser class 3B
– Diode laser (SHG) 561nm, 10mW, laser
class 3B
– Diode laser 640nm, 5mW, laser class 3B
Directly modulated, dynamic range 500:1.

High Resolution Digital Microscopy Camera: AxioCam 503 mono

Camera Adapter 60N-C 2/3″ 0.63x

Microscopy Camera Axiocam 503 mono (D)

High Performance microscopy camera incl. driver software, USB 3.0 PCIe x1 interface, dual USB 3.0/USB 2.0 cable 3 m and BK7 protection glass (coated)
Number of Pixels:  1936 (H) x 1460 (V) = 2.8 Mega Pixels;Pixel size:  4.54 µm x 4.54 µm; Chip size:  8.8 mm x 6.6 mm, equivalent to 2/3″ (11 mm diagonal)
Spectral range:  With protection glass app. 400 nm to 1000 nm
CE Certified