Cytiva is an established component provider for point-of-care immunodiagnostic assays, covering confirmatory Western blots and line assays and specifically lateral flow and flow-through immunoassays. We produce a vast array of cellulose and glass fiber substrates and nitrocellulose membranes to an assured quality, ensuring accurate and reproducible results. Our diverse array of products for lateral flow technology includes our wide range of blood separation products, conjugate release pads, nitrocellulose membranes, and absorbents.
Single-step lateral flow immunoassays require only the addition of a sample. The sample flows through the device and comes in contact with dried reagents, usually a tagged secondary antibody. The antibody and analyte migrate to a capture zone with membrane-immobilized antibody or antigen. Any unreacted tagged antibody flows past the capture zone. In a flow-through immunoassay the sample is applied directly to the membrane surface and is allowed to wick through the membrane into an absorbent paper below.